LINUS PAULING INSTITUTE RESEARCH REPORT


Cancer Chemoprotection Program (CCP) Update


It has been an exciting year for the LPI CCP. The number of faculty associated with the program has doubled and now includes Drs. Rod Dashwood (Chief), David Williams, George Bailey, Rosita Rodriguez, Emily Ho, and Gayle Orner. Additionally, Dr. David Yu was hired to manage the CCP Core Laboratory.

Established in April 2002 by a generous gift from an LPI donor, the CCP Core Laboratory at LPI provides genetic toxicology testing services. The mission of this laboratory is to identify dietary compounds with potential cancer chemoprotective activities and to elucidate the inhibitory mechanisms. The major assays currently employed are the Salmonella mutagenicity assay (or Ames test), single cell gel electrophoresis assay (SCG or Comet assay), and micronucleus assay. These assays enable investigators to assess the mutagenic, DNA-damaging, and clastogenic effects of chemicals. Using known mutagens or clastogens (chromosome damaging substances) as “positive controls”, the tests are conducted to identify the potential beneficial activities of dietary compounds. Specifically, the Ames test is used to determine the anti-mutagenicity of compounds, including protective dietary substances. The Comet assay is used to identify compounds that prevent DNA strand breaks in whole cells. Protection against chromosome damage is assessed using the micronucleus assay.

Dr. David Yu manages the CCP Core Laboratory and oversees the various assays. While these services are used routinely by LPI and OSU researchers, they are also available to other professionals. For more information, please contact Dr. David Yu at LPI.

Last updated November, 2003


Micronutrient Research for Optimum Health


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