Mass Spectrometry
Analysis of samples by HPLC-tandem mass
spectrometry performed by the ONSL staff
Isoprostanes are stereoisomers of prostaglandin F2-alpha and are products of free radical-induced oxidation of arachidonic acid. They are considered to be good biomarkers for oxidative stress.
Quantitation in plasma of total (free + esterified) 15-series isoprostanes, including 8-iso-PGF2 alpha, is currently performed by the procedure described in Taylor et al. (2006) Analytical Biochemistry 350, 41-51. The service is free to LPI investigators or at a cost of $278.37 per sample to other researchers. Arachidonic acid can also be quantified in the same sample ($38.50 per sample for outside investigators).
The assay is being extended to the analysis of free isoprostanes in plasma.
A new assay is being validated for the analysis of free isoprostanes and isoprostane metabolites in urine.
Submit samples and instructions for sample preparation and shipping.
Tandem mass spectrometry coupled to HPLC is a powerful tool for biomarker discovery and quantitation. The instrument, operated by ONSL staff, is available for custom analyses. Recent work includes development of methods for studying ceramides and other sphingolipids and improved sensitivity for tocopherol assay.
Solid phase extraction (SPE) can improve analytical results by producing cleaner extracts of many analytes found in biological samples. ONSL has recently obtained two robots to perform SPE and can assist with development of new sample preparation methods.
Please contact Alan Taylor (541-737-9376 or Alan.Taylor@oregonstate.edu) for advice on suitability of use, scheduling, and sample preparation.
