Mass Spectometry Samples
The plasma total isoprostane assay requires 0.5 mL of sample, but 1 mL or greater volumes are encouraged. An additional 0.1 mL of plasma is needed for the arachidonic assay.
Preparation of samples
Blood should be drawn in a manner which avoids or minimizes hemolysis: use of larger needles (21 gauge minimum) and gentle suction while drawing blod from humans or large animals, capture of free flowing blood from sacrificed small animals, and gentle handling of filled tubes are suggested.
EDTA is the preferred preservative/anticoagulant, but heparin may also be used.
Fresh blood should then quickly be centrifuged (500 x g) to produce plasma clear of cells or visible debris.
Plasma should be aliquoted into screw-capped cryovials, leaving space for expansion, then flash-frozen in liquid nitrogen and stored at -80 degrees C until shipping.
Use a permanent marker to directly label vials, backing up "stick-on" labels.
Please contact the ONSL manager (Alan.Taylor@oregonstate.edu or 541-737-9376) before shipping your samples.
After acknowledgement, please ship by overnight delivery with sufficient dry ice to ensure samples remain well frozen.
Sample cryovials should be packed in a box secured with string and placed inside a tied or sealed plastic bag. Include a complete sample list.
Please send the delivery information to the manager to ensure proper handling upon arrival.
Assays will be scheduled upon receipt of the samples and the analytical services agreement.