The CCP Core Laboratory provides genetic toxicology testing services to help investigators identify dietary compounds with chemoprotective properties and to elucidate their inhibitory mechanism(s). Techniques include the Salmonella mutagenicity assay (Ames test), single-cell gel electrophoresis assay (Comet assay), and micronucleus assay, which enable investigators to assess the mutagenic, DNA-damaging, and clastogenic effects of chemicals. Using known mutagens or clastogens as positive controls, we study the potential beneficial activities of dietary compounds or other chemicals against those DNA-damaging agents. A histone deacetylase (HDAC) assay is available to identify dietary HDAC inhibitors, which are of interest in cancer chemoprotection.
These services are available to intramural or extramural (non-OSU) researchers. Please contact Dr. David Yu, CCP Lab Manager (David.Yu@oregonstate.edu or 541-737-5082).
Oxidative and nitrative stress can be produced by inflammation, disease, or environmental exposure. The resulting reactive oxygen and nitrogen species can react with lipids, proteins, and nucleic acids to form molecules that can serve as “biomarkers” to reveal an individual’s state of health or the effectiveness of dietary or pharmaceutical interventions. For example, F2-isoprostanes and their metabolites are considered good biomarkers of free radical-induced lipid peroxidation. The ONSL has several high-performance liquid chromatographs (HPLCs) coupled to UV, fluorescence, electrochemical, and single- and triple-quadrupole mass spectrometer detectors for state-of-the-art chemical analysis of biomarkers, antioxidants, and drug metabolites. ONSL also has several solid-phase extraction robots for sample preparation.
These services are available to intramural and extramural investigators. Please contact Jaewoo Choi, ONSL Manager (email@example.com or 541-737-9376).
The Food Composition Laboratory provides chemical analyses of food composition and nutritional factors. We provide analyses of food, feed, or dietary supplement samples for vitamins, phytochemicals, and other compositional factors to intramural and extramural investigators.
Please contact Bob Durst (Bob.Durst@oregonstate.edu or 541-737-6490).