Title1α,25-dihydroxyvitamin D-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression.
Publication TypeJournal Article
Year of Publication2018
AuthorsJiang J, Zhang Y, Indra AK, Ganguli-Indra G, Le MN, Wang H, Hollins RR, Reilly DA, Carlson MA, Gallo RL, Gombart AF, Xie J
JournalNanomedicine (Lond)
Volume13
Issue12
Pagination1417-1432
Date Published2018 06
ISSN1748-6963
KeywordsAnimals, Anti-Infective Agents, Antimicrobial Cationic Peptides, Bandages, Cell Line, Humans, Keratinocytes, Mice, Mice, Transgenic, Monocytes, Nanofibers, Vitamin D, Wound Closure Techniques
Abstract

AIM: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D (1,25(OH)D) and augmenting human CAMP induction.

MATERIALS & METHODS: Nanofibrous wound dressings containing 1,25(OH)D were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.

RESULTS: 1,25(OH)D was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)D showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)D-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants.

CONCLUSION: 1,25(OH)D containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.
DOI10.2217/nnm-2018-0011
Alternate JournalNanomedicine (Lond)
PubMed ID29972648
PubMed Central IDPMC6219435
Grant ListP20 GM103480 / GM / NIGMS NIH HHS / United States
R01 GM123081 / GM / NIGMS NIH HHS / United States
2P20 GM103480-06 / NH / NIH HHS / United States
1R01GM123081 / NH / NIH HHS / United States