TitleA 60-kDa polypeptide in mammalian cells with epitopes related to actin.
Publication TypeJournal Article
Year of Publication1987
AuthorsVarma M, Aebi U, Fleming J, Leavitt J
JournalExp Cell Res
Date Published1987 Nov
KeywordsActins, Animals, Anti-Bacterial Agents, Antibodies, Monoclonal, Deoxyribonuclease I, Epitopes, Humans, Immunosorbent Techniques, Isoelectric Point, Macrolides, Mitochondria, Molecular Weight, Rats, Solubility

We have identified a novel actin-related 60-kDa polypeptide in mammalian cells. The relatedness of this polypeptide to actin is indicated by its affinity for DNase I, two monoclonal anti-actin antibodies, and two independent peptide-specific anti-actin antibodies which bind to actin at around amino acid 244. It is not incorporated into cytoskeletal stress fibers, although it is a stable protein. Its expression (60-kDa polypeptide, pI of 5.4 to 5.5) is inhibited by the K+ ionophore, nonactin, which is known to collapse the energy-dependent translocation of cytoplasmically synthesized proteins into mitochondria.

Alternate JournalExp. Cell Res.
PubMed ID2445592
Grant ListCA34763 / CA / NCI NIH HHS / United States
GM35171 / GM / NIGMS NIH HHS / United States