OREGON STATE UNIVERSITY
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| Title | A 60-kDa polypeptide in mammalian cells with epitopes related to actin. |
| Publication Type | Journal Article |
| Year of Publication | 1987 |
| Authors | Varma M, Aebi U, Fleming J, Leavitt J |
| Journal | Exp Cell Res |
| Volume | 173 |
| Issue | 1 |
| Pagination | 163-73 |
| Date Published | 1987 Nov |
| ISSN | 0014-4827 |
| Keywords | Actins, Animals, Anti-Bacterial Agents, Antibodies, Monoclonal, Deoxyribonuclease I, Epitopes, Humans, Immunosorbent Techniques, Isoelectric Point, Macrolides, Mitochondria, Molecular Weight, Rats, Solubility |
| Abstract | We have identified a novel actin-related 60-kDa polypeptide in mammalian cells. The relatedness of this polypeptide to actin is indicated by its affinity for DNase I, two monoclonal anti-actin antibodies, and two independent peptide-specific anti-actin antibodies which bind to actin at around amino acid 244. It is not incorporated into cytoskeletal stress fibers, although it is a stable protein. Its expression (60-kDa polypeptide, pI of 5.4 to 5.5) is inhibited by the K+ ionophore, nonactin, which is known to collapse the energy-dependent translocation of cytoplasmically synthesized proteins into mitochondria. |
| DOI | 10.1016/0014-4827(87)90342-9 |
| Alternate Journal | Exp. Cell Res. |
| PubMed ID | 2445592 |
| Grant List | CA34763 / CA / NCI NIH HHS / United States GM35171 / GM / NIGMS NIH HHS / United States |