TitleAssessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure.
Publication TypeJournal Article
Year of Publication2015
AuthorsWatson GW, Wickramasekara S, Maier CS, Williams DE, Dashwood RH, Ho E
JournalEuPA Open Proteom
Date Published2015 Dec

The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.

Alternate JournalEuPA Open Proteom
PubMed ID26640761
PubMed Central IDPMC4666544
Grant ListP01 CA090890 / CA / NCI NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States