TitleAssessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure.
Publication TypeJournal Article
Year of Publication2015
AuthorsWatson GW, Wickramasekara S, Maier CS, Williams DE, Dashwood RH, Ho E
JournalEuPA Open Proteom
Volume9
Pagination34-40
Date Published2015 Dec
ISSN2212-9685
Abstract

The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.

DOI10.1016/j.euprot.2015.08.002
Alternate JournalEuPA Open Proteom
PubMed ID26640761
PubMed Central IDPMC4666544
Grant ListP01 CA090890 / CA / NCI NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States