Title | Assessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Watson GW, Wickramasekara S, Maier CS, Williams DE, Dashwood RH, Ho E |
Journal | EuPA Open Proteom |
Volume | 9 |
Pagination | 34-40 |
Date Published | 2015 Dec |
ISSN | 2212-9685 |
Abstract | The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells. |
DOI | 10.1016/j.euprot.2015.08.002 |
Alternate Journal | EuPA Open Proteom |
PubMed ID | 26640761 |
PubMed Central ID | PMC4666544 |
Grant List | P01 CA090890 / CA / NCI NIH HHS / United States P30 ES000210 / ES / NIEHS NIH HHS / United States |