TitleBurn and smoke inhalation injury in sheep depletes vitamin E: kinetic studies using deuterated tocopherols.
Publication TypeJournal Article
Year of Publication2007
AuthorsTraber MG, Shimoda K, Murakami K, Leonard SW, Enkhbaatar P, Traber LD, Traber DL
JournalFree Radic Biol Med
Date Published2007 May 01
KeywordsAnimals, Burns, Deuterium, Disease Models, Animal, Kinetics, Sheep, Smoke, Smoking, Time Factors, Vitamin E, Vitamin E Deficiency, Wakefulness

To test the hypothesis that burn and smoke injury will deplete tissue alpha-tocopherol and cause its faster plasma disappearance, deuterium-labeled vitamin E was administered to sheep exposed to both surface skin burn and smoke insufflation, which cause injuries similar to those of human victims of fire accidents. Two different protocols were used: (1) deuterated vitamin E was administered orally with food at time 0 (just before injury) or (2) the labeled vitamin E was administered orally with food the day before injury. The animals, which had been operatively prepared seven days before, were anesthetized and then received both 40% body surface area third-degree burn and 48 breaths of cotton smoke or sham injuries. All were resuscitated with Ringer's lactate solution (4 ml/kg/% BSA burn/24 h) and mechanically ventilated. Blood samples were collected at various times after vitamin E dosing. In both studies the depletion of plasma alpha-tocopherol was faster in the injured sheep. The sheep given deuterated vitamin E 24 h before injury had similar maximum alpha-tocopherol concentrations at similar times. The exponential rates of alpha-tocopherol disappearance were 1.5 times greater and half-lives were 12 h shorter (p < 0.05) in the injured sheep. In separate studies, various tissues were obtained from sheep that were sacrificed from 4 to 48 h after injury. The liver alpha-tocopherol concentrations in sheep killed at various times after injury seem to show a linear decrease at a rate of 0.1 nmol alpha-tocopherol/g liver per hour, suggesting that the liver is supplying alpha-tocopherol to maintain the plasma and lung alpha-tocopherol concentrations, but that this injury is so severe the liver is unable to maintain lung alpha-tocopherol concentrations. These findings suggest that alpha-tocopherol should be administered to burn patients to prevent vitamin E depletion and to protect against oxidative stress from burn injury.

Alternate JournalFree Radic. Biol. Med.
PubMed ID17395015
PubMed Central IDPMC1899466
Grant ListR01 GM060688 / GM / NIGMS NIH HHS / United States
GM60688 / GM / NIGMS NIH HHS / United States
P01 GM066312-03 / GM / NIGMS NIH HHS / United States