TitleElectrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats.
Publication TypeJournal Article
Year of Publication2013
AuthorsWickramasekara SI, Zandkarimi F, Morré J, Kirkwood J, Legette LC, Jiang Y, Gombart AF, Stevens JF, Maier CS
Date Published2013 Aug 13

This study reports on the use of traveling wave ion mobility quadrupole time-of-flight (ToF) mass spectrometry for plasma metabolomics. Plasma metabolite profiles of obese Zucker fa/fa rats were obtained after the administration of different oral doses of Xanthohumol; a hop-derived dietary supplement. Liquid chromatography coupled data independent tandem mass spectrometry (LC-MSE) and LC-ion mobility spectrometry (IMS)-MSE acquisitions were conducted in both positive and negative modes using a Synapt G2 High Definition Mass Spectrometry (HDMS) instrument. This method provides identification of metabolite classes in rat plasma using parallel alternating low energy and high energy collision spectral acquisition modes. Data sets were analyzed using pattern recognition methods. Statistically significant (p < 0.05 and fold change (FC) threshold > 1.5) features were selected to identify the up-/down-regulated metabolite classes. Ion mobility data visualized using drift scope software provided a graphical read-out of differences in metabolite classes.

Alternate JournalMetabolites
PubMed ID24958146
PubMed Central IDPMC3901285
Grant ListP30 ES000210 / ES / NIEHS NIH HHS / United States
R01 AI065604 / AI / NIAID NIH HHS / United States
R21 AT005294 / AT / NCCIH NIH HHS / United States
S10 RR025628 / RR / NCRR NIH HHS / United States