TitleEngineered Exosomes Containing Cathelicidin/LL-37 Exhibit Multiple Biological Functions.
Publication TypeJournal Article
Year of Publication2022
AuthorsSu Y, Sharma NShree, John JV, Ganguli-Indra G, Indra AK, Gombart AF, Xie J
JournalAdv Healthc Mater
Volume11
Issue20
Paginatione2200849
Date Published2022 Oct
ISSN2192-2659
KeywordsAntimicrobial Cationic Peptides, Biocompatible Materials, Cathelicidins, Exosomes, Human Umbilical Vein Endothelial Cells, Humans, Vitamin D3 24-Hydroxylase
Abstract

Exosomes show great potential in diagnostic and therapeutic applications. Inspired by the human innate immune defense, herein, we report engineered exosomes derived from monocytic cells treated with immunomodulating compounds 1α,25-dihydroxyvitamin D and CYP24A1 inhibitor VID400 which are slowly released from electrospun nanofiber matrices. These engineered exosomes contain significantly more cathelicidin/LL-37 when compared with exosomes derived from either untreated cells or Cathelicidin Human Tagged ORF Clone transfected cells. In addition, such exosomes exhibit multiple biological functions evidenced by killing bacteria, facilitating human umbilical vein endothelial cell tube formation, and enhancing skin cell proliferation and migration. Taken together, the engineered exosomes developed in this study can be used as therapeutics alone or in combination with other biomaterials for effective infection management, wound healing, and tissue regeneration.
DOI10.1002/adhm.202200849
Alternate JournalAdv Healthc Mater
PubMed ID35930707
PubMed Central IDPMC9588668
Grant ListR01 GM123081 / GM / NIGMS NIH HHS / United States