Title | Germ-Free Swiss Webster Mice on a High-Fat Diet Develop Obesity, Hyperglycemia, and Dyslipidemia. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Logan IE, Bobe G, Miranda CL, Vasquez-Perez S, Choi J, Lowry MB, Sharpton TJ, Morgun A, Maier CS, Stevens JF, Shulzhenko N, Gombart AF |
Journal | Microorganisms |
Volume | 8 |
Issue | 4 |
Date Published | 2020 Apr 05 |
ISSN | 2076-2607 |
Abstract | A calorie-dense diet is a well-established risk factor for obesity and metabolic syndrome (MetS), whereas the role of the intestinal microbiota (IMB) in the development of diet-induced obesity (DIO) is not completely understood. To test the hypothesis that Swiss Webster (Tac:SW) mice can develop characteristics of DIO and MetS in the absence of the IMB, we fed conventional (CV) and germ-free (GF) male Tac:SW mice either a low-fat diet (LFD; 10% fat derived calories) or a high-fat diet (HFD; 60% fat derived calories) for 10 weeks. The HFD increased feed conversion and body weight in GF mice independent of the increase associated with the microbiota in CV mice. In contrast to CV mice, GF mice did not decrease feed intake on the HFD and possessed heavier fat pads. The HFD caused hyperglycemia, hyperinsulinemia, and impaired glucose absorption in GF mice independent of the increase associated with the microbiota in CV mice. A HFD also elevated plasma LDL-cholesterol and increased hepatic triacylglycerol, free fatty acids, and ceramides in all mice, whereas hypertriglyceridemia and increased hepatic medium and long-chain acylcarnitines were only observed in CV mice. Therefore, GF male Tac:SW mice developed several detrimental effects of obesity and MetS from a high-fat, calorie dense diet. |
DOI | 10.3390/microorganisms8040520 |
PubMed ID | 32260528 |
PubMed Central ID | PMC7232377 |
Grant List | 1R01DK103761-01A1 / NH / NIH HHS / United States 5R01AT009168 / NH / NIH HHS / United States 2017-18 Christopher and Catherine Mathews Graduate Fellowship / / Oregon State University / S10RR027878 / NH / NIH HHS / United States 2019 Audrey & George Varsevelt LPI Graduate Fellowship / / Oregon State University / 2019-20 Mark Spoonenburgh LPI Graduate Fellowship / / Oregon State University / R01 DK103761 / DK / NIDDK NIH HHS / United States |