TitleGut enterotype-dependent modulation of gut microbiota and their metabolism in response to xanthohumol supplementation in healthy adults.
Publication TypeJournal Article
Year of Publication2024
AuthorsJamieson PE, Smart EB, Bouranis JA, Choi J, Danczak RE, Wong CP, Paraiso IL, Maier CS, Ho E, Sharpton TJ, Metz TO, Bradley R, Stevens JF
JournalGut Microbes
Date Published2024 Jan-Dec
KeywordsAdult, Flavonoids, Gastrointestinal Microbiome, Humans, Prebiotics, Propiophenones, RNA, Ribosomal, 16S

Xanthohumol (XN), a polyphenol found in the hop plant (), has antioxidant, anti-inflammatory, prebiotic, and anti-hyperlipidemic activity. Preclinical evidence suggests the gut microbiome is essential in mediating these bioactivities; however, relatively little is known about XN's impact on human gut microbiota . We conducted a randomized, triple-blinded, placebo-controlled clinical trial (ClinicalTrials.gov NCT03735420) to determine safety and tolerability of XN in healthy adults. Thirty healthy participants were randomized to 24 mg/day XN or placebo for 8 weeks. As secondary outcomes, quantification of bacterial metabolites and 16S rRNA gene sequencing were utilized to explore the relationships between XN supplementation, gut microbiota, and biomarkers of gut health. Although XN did not significantly change gut microbiota composition, it did re-shape individual taxa in an enterotype-dependent manner. High levels of inter-individual variation in metabolic profiles and bioavailability of XN metabolites were observed. Moreover, reductions in microbiota-derived bile acid metabolism were observed, which were specific to and enterotypes. These results suggest interactions between XN and gut microbiota in healthy adults are highly inter-individualized and potentially indicate that XN elicits effects on gut health in an enterotype-dependent manner.

Alternate JournalGut Microbes
PubMed ID38358253
PubMed Central IDPMC10878022
Grant ListR01 AT010271 / AT / NCCIH NIH HHS / United States
S10 OD026922 / OD / NIH HHS / United States
S10 RR022589 / RR / NCRR NIH HHS / United States
S10 RR027878 / RR / NCRR NIH HHS / United States