Title | The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Miller GW, Truong L, Barton CL, Labut EM, Lebold KM, Traber MG, Tanguay RL |
Journal | Comp Biochem Physiol Part D Genomics Proteomics |
Volume | 10 |
Pagination | 22-9 |
Date Published | 2014 Jun |
ISSN | 1878-0407 |
Keywords | Animals, Diet, Embryo, Nonmammalian, Female, Gene Expression Regulation, Developmental, Male, Transcriptome, Vitamin E, Zebrafish |
Abstract | The composition of the typical commercial diet fed to zebrafish can dramatically vary. By utilizing defined diets we sought to answer two questions: 1) How does the embryonic zebrafish transcriptome change when the parental adults are fed a commercial lab diet compared with a sufficient, defined diet (E+)? 2) Does a vitamin E-deficient parental diet (E-) further change the embryonic transcriptome? We conducted a global gene expression study using embryos from zebrafish fed a commercial (Lab), an E+ or an E- diet. To capture differentially expressed transcripts prior to onset of overt malformations observed in E- embryos at 48h post-fertilization (hpf), embryos were collected from each group at 36hpf. Lab embryos differentially expressed (p<0.01) 946 transcripts compared with the E+ embryos, and 2656 transcripts compared with the E- embryos. The differences in protein, fat and micronutrient intakes in zebrafish fed the Lab compared with the E+ diet demonstrate that despite overt morphologic consistency, significant differences in gene expression occurred. Moreover, functional analysis of the significant transcripts in the E- embryos suggested perturbed energy metabolism, leading to overt malformations and mortality. Thus, these findings demonstrate that parental zebrafish diet has a direct impact on the embryonic transcriptome. |
DOI | 10.1016/j.cbd.2014.02.001 |
Alternate Journal | Comp. Biochem. Physiol. Part D Genomics Proteomics |
PubMed ID | 24657723 |
PubMed Central ID | PMC4037372 |
Grant List | P30 ES000210 / ES / NIEHS NIH HHS / United States R01 HD062109 / HD / NICHD NIH HHS / United States RC4 ES019764 / ES / NIEHS NIH HHS / United States |