TitleIsolation of chlorophylls a and b from spinach by counter-current chromatography.
Publication TypeJournal Article
Year of Publication2007
AuthorsJubert C, Bailey G
JournalJ Chromatogr A
Volume1140
Issue1-2
Pagination95-100
Date Published2007 Jan 26
ISSN0021-9673
KeywordsChlorophyll, Chlorophyll A, Chromatography, High Pressure Liquid, Countercurrent Distribution, Nuclear Magnetic Resonance, Biomolecular, Spectrometry, Mass, Fast Atom Bombardment, Spinacia oleracea
Abstract

A method for the isolation of chlorophylls from spinach by counter-current chromatography was developed. An initial extraction protocol was devised to avoid the notorious sensitivity of chlorophylls to degradation by light, heat, oxygen, acids and bases. Further purification and separation of chlorophylls a and b were achieved using counter-current chromatography. Chlorophyll structures and purities were established by HPLC, fast atom bombardment mass spectrometry and nuclear magnetic resonance. Purity was estimated to be >95% (100% by HPLC). Typical yields from 30g of freeze-dried spinach were 300mg of chlorophyll a and 100mg of chlorophyll b.
DOI10.1016/j.chroma.2006.11.063
Alternate JournalJ Chromatogr A
PubMed ID17164074
Grant ListES00210 / ES / NIEHS NIH HHS / United States
ES03850 / ES / NIEHS NIH HHS / United States
P01 CA90890 / CA / NCI NIH HHS / United States