|Title||Liver ascorbate and glutathione disulfide levels in guinea pigs are affected by dietary ascorbate intake and the type of diet.|
|Publication Type||Journal Article|
|Year of Publication||1995|
|Authors||Netke S, Niedzwiecki A|
|Journal||Int J Vitam Nutr Res|
|Keywords||Animals, Ascorbic Acid, Cholesterol, Dietary, Dehydroascorbic Acid, Diet, Dietary Carbohydrates, Dietary Fats, Glutathione, Glutathione Disulfide, Guinea Pigs, Lipid Peroxidation, Liver, Oxidation-Reduction, Thiobarbituric Acid Reactive Substances|
We studied effects on the levels and oxidant status of AA and GSH in the guinea pig liver. Group I received various doses of AA supplementation in a standard diet, Group II in a "western" high fat, high sugar, high cholesterol diet. Both diets were supplemented with 1 (Ia and IIa); 25, (Ib and IIb); and 1000, (Ic and IIc) mg AA per animal per day. The Group II animals were sacrificed during the 9th and 10th weeks, those in Group I during the 19th and 20th weeks. Liver AA levels were 30 and 26 micrograms/g in subgroups Ia and IIa, 367 and 282 micrograms/g in subgroups Ib and IIb, and 604 and 481 micrograms/g in subgroups Ic and IIc. In subgroups Ia and IIa most of the AA was detected as DHAA. DHAA and AA levels were related in a biphasic manner. GSSG content tended to increase with higher intakes of AA regardless of diet and ranged between 0.094 to 0.194 mumol/g. GSH levels were not affected by diet type or AA level. GSSG and DHAA levels were affected only by dietary AA. Changes in serum TBARS suggested a preventive effect of higher dietary ascorbate on lipid peroxidation, but only with a western type diet.
|Alternate Journal||Int J Vitam Nutr Res|