TitleMeasurement of the vitamin E metabolites, carboxyethyl hydroxychromans (CEHCs), in biological samples.
Publication TypeJournal Article
Year of Publication2006
AuthorsLeonard SW, Traber MG
JournalCurr Protoc Toxicol
VolumeChapter 7
PaginationUnit7.8
Date Published2006 Sep
ISSN1934-9262
KeywordsAnimals, Chromans, Chromatography, High Pressure Liquid, Humans, Mass Spectrometry, Propionates, Vitamin E
Abstract

Metabolites of α- and γ-tocopherol, 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (α-CEHC) and 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxychroman (γ-CEHC), respectively, are produced in the liver and have been measured in biological fluids and tissue. Compared to α-tocopherol concentrations, metabolite concentrations are as much as a factor of a thousand lower, requiring extremely sensitive methodology to attain accurate measurements. This unit presents a protocol for CEHC extraction from biological samples, and describes very specific and sensitive HPLC/MS analysis.
DOI10.1002/0471140856.tx0708s29
Alternate JournalCurr Protoc Toxicol
PubMed ID20954161