TitleMechanistic aspects of ascorbate inhibition of human immunodeficiency virus.
Publication TypeJournal Article
Year of Publication1994
AuthorsHarakeh S, Niedzwiecki A, Jariwalla RJ
JournalChem Biol Interact
Date Published1994 Jun
KeywordsAscorbic Acid, beta-Galactosidase, Blotting, Northern, Cell Line, Cell Survival, Genes, Reporter, HIV, HIV Long Terminal Repeat, Humans, Protein Biosynthesis, Reverse Transcriptase Inhibitors, RNA, Viral, Transcription, Genetic, Viral Proteins

We have investigated the molecular basis of the inhibitory effect of ascorbate (vitamin C) on human immunodeficiency virus (HIV) expression in unstimulated chronically infected and reporter cell lines. Comparison of intracellular HIV RNA and protein patterns of ascorbate-treated cells with corresponding patterns of untreated controls, did not show significant differences in the synthesis or processing of individual viral RNA and polypeptides, indicating that the inhibitory effect of ascorbate is not directed at steps of viral transcription or translation. Enzyme assays on cell extracts showed that the activity of an HIV LTR-directed reporter protein made in ascorbate-treated cells was reduced to approximately 11% relative to that of untreated control. These results, combined with previous observations on the suppression of HIV RT activity, are consistent with a mechanism of action in which ascorbate exerts a posttranslational inhibitory effect on HIV by causing impairment of enzymatic activity.

Alternate JournalChem. Biol. Interact.
PubMed ID7514961