TitlePhytochemical characterization and bioactivity toward breast cancer cells of unhydrolyzed and acid-hydrolyzed extracts of .
Publication TypeJournal Article
Year of Publication2022
AuthorsMiranda CL, Kumbi Y, Wu W, Lee H-S, Reed RL, Stevens JF
JournalNat Prod Commun
Date Published2022 Jul

Phytochemicals from the genus, , have been attracting increasing attention due to their potential beneficial effects on human health. species contain various types of phytochemicals such as flavonoids, alkaloids, saponins, terpenoids, coumarins and tannins. In this study, we investigated the phytochemical composition of unhydrolyzed and acid-hydrolyzed extracts of and their bioactivity toward breast cancer MCF-7 cells in vitro. The results revealed that contains phytochemicals consistent with the reported phytochemical composition of this species, with greater amounts of aglycones detected in the hydrolyzed extract. The crude extract of without acid hydrolysis was found to be ineffective in inhibiting the growth of MCF-7 cells at doses below 1000 μg/mL. However, after acid hydrolysis (to mimic gastro-intestinal hydrolysis), the extract became growth-inhibitory to MCF-7 cells as low as 10 μg/mL and the cytotoxicity increased with increasing dose and time of treatment. The results suggest that extracts contain phytochemicals in glycosidic forms whose aglycones are active as anti-proliferative agents toward breast cancer cells in vitro.

Alternate JournalNat Prod Commun
PubMed ID35875707
PubMed Central IDPMC9302922
Grant ListS10 RR027878 / RR / NCRR NIH HHS / United States