TitlePhytochemical Investigation and Reproductive Capacity of the Bulgarian Endemic Plant Species Boiss. (Lamiaceae).
Publication TypeJournal Article
Year of Publication2021
AuthorsZheljazkov VD, Semerdjieva IB, Stevens JF, Wu W, Cantrell CL, Yankova-Tsvetkova E, Koleva-Valkova LH, Stoyanova A, Astatkie T
JournalPlants (Basel)
Date Published2021 Dec 30

Boiss (Lamiaceae) is a Bulgarian endemic species. Overall, the essential oil (EO) composition of was different from that of the other species reported in the literature. The main EO constituents of were ()-caryophyllene, germacrene D, and caryophyllene oxide. The effect of the harvest stage was significant only on α-copaene, ()-caryophyllene, caryophyllene oxide, and τ-muurolol. The concentration of α-copaene (1.26-1.83% range of the total oil), ()-caryophyllene (31-41%), caryophyllene oxide (6.4-11.8%), and τ-muurolol (1.3-2.8%) were the highest at 2-3 pair of leaves or before flowering and lower at flowering. The harvest stage did not significantly affect the concentrations of the other six identified EO compounds β-bourbonene (1.1%), α-humulene (2.8%), germacrene D (23.3%), bicyclogermacrene (2.85%), δ-cadinene (1.1%), and spathulenol (2.8%). In a separate experiment, grinding of the biomass prior to EO extraction had a significant effect only on the concentrations of D-limonene (0.24-3.3%) and bicyclogermacrene (3.6-9.1%). Grinding in water or without water, maceration, and addition of Tween20 had rather small effects on the EO profile. The identified EO constituents and their mean concentrations in this experiment were ()-caryophyllene (25.4%), germacrene D (17.6%), caryophyllene oxide (9.1%), spathulenol (6.5%), τ-muurolol (5.0%), carvacrol (3.9%), α-copaene (2.5%), β-bourbonene (2.5%), δ-cadinene (2.4%), α-humulene (1.8%), and Z-β-farnesene (1.3%). Embryological studies observed anther and the development of the male gametophyte and ovule and development of the female gametophyte of . Furthermore, pollen and seed viability assays were conducted, and mass spectrometry-based metabolomics analysis of an extract from shoots revealed the presence of 45 natural products, identified as flavonoids, phenolic acids, and (tri)terpenoids. Overall, the phytochemistry and some of the microscopic analyses distinguished this endemic species from other species in .

Alternate JournalPlants (Basel)
PubMed ID35009117
PubMed Central IDPMC8747201
Grant ListS10 RR027878 / RR / NCRR NIH HHS / United States