TitlePyrrolidine dithiocarbamate is a potent antioxidant against hypochlorous acid-induced protein damage.
Publication TypeJournal Article
Year of Publication2002
AuthorsZhu B-Z, Carr AC, Frei B
JournalFEBS Lett
Volume532
Issue1-2
Pagination80-4
Date Published2002 Dec 04
ISSN0014-5793
KeywordsAcetylcysteine, Aldehydes, alpha 1-Antitrypsin, Antioxidants, Chlorine, Cyclohexanones, Dose-Response Relationship, Drug, Enzyme Inhibitors, Ferrocyanides, Glutathione, Hypochlorous Acid, Lipoproteins, LDL, Oxidants, Oxidation-Reduction, Oxidative Stress, Pyrrolidines, Serum Albumin, Thiocarbamates
Abstract

The antioxidant potential of the dithiol compound pyrrolidine dithiocarbamate (PDTC) against protein damage induced by hypochlorous acid (HOCl) was investigated. The effects of PDTC were compared to those of reduced glutathione (GSH) and N-acetylcysteine (NAC). PDTC markedly and in a concentration-dependent manner inhibited HOCl-induced inactivation of alpha(1)-antiproteinase, protein carbonyl formation on serum albumin and oxidation of human low-density lipoprotein. The direct scavenging of HOCl by PDTC was demonstrated by two quantitative methods, oxidation of ferrocyanide and chlorination of monochlorodimedon. In all assay systems, PDTC was two to three times more potent than GSH and NAC, while diethyldithiocarbamate was about as effective as PDTC. These data demonstrate that PDTC is a potent antioxidant against HOCl-induced protein oxidative damage, suggesting that PDTC might be useful in the prevention and treatment of inflammatory conditions.
DOI10.1016/s0014-5793(02)03637-2
Alternate JournalFEBS Lett.
PubMed ID12459467
Grant ListAT00066 / AT / NCCIH NIH HHS / United States
ES00210 / ES / NIEHS NIH HHS / United States
ES11497 / ES / NIEHS NIH HHS / United States
HL60886 / HL / NHLBI NIH HHS / United States