Title | Quantification of Caffeoylquinic Acids and Triterpenes as Targeted Bioactive Compounds of in Extracts and Formulations by Liquid Chromatography Mass Spectrometry. |
Publication Type | Journal Article |
Year of Publication | 2023 |
Authors | Yang L, Marney L, Magana AAlcazar, Choi J, Wright K, Mcferrin J, Gray NE, Soumyanath A, Stevens JF, Maier CS |
Journal | J Chromatogr Open |
Volume | 4 |
Date Published | 2023 Nov 27 |
ISSN | 2772-3917 |
Abstract | (CA) is a culinary vegetable and well-known functional food that is widely used as a medicinal herb and dietary supplement. CA is rich in pentacyclic triterpenes (TTs), including asiaticoside (AS), madecassoside (MS) and the related aglycones asiatic acid (AA), madecassic acid (MA). Traditionally, TTs have been associated with the bioactivity and health promoting effect of CA. Recently, mono-caffeoylquinic acids (MonoCQAs) and di-caffeoylquinic acids (DiCQAs) have been found to contribute to the bioactivity of CA as well. This work reports an analytical strategy based on liquid chromatography coupled to multiple reaction monitoring mass spectrometry (LC-MRM-MS) for the simultaneous rapid and accurate quantification of 12 bioactive compounds in CA, namely AS, MS, AA, MA, 5-CQA, 4-CQA, 3-CQA, 1,3-DiCQA, 3,4-DiCQA, 1,5-DiCQA, 3,5-DiCQA, 4,5-DiCQA. Method selectivity, accuracy, precision, repeatability, robustness, linearity range, limit of detection (LOD), and limit of quantitation (LOQ) were validated. The validated LC-MRM-MS method has been successfully applied to quantify the 12 bioactive compounds in CA aqueous extracts and two related formulations: a standardized CA product (CAP) used in a phase I clinical trial and formulated CA rodent diets used in preclinical studies. The validated method allows us to support the standardization of CA products used for clinical trials and conduct routine LC-MRM-MS analyses of formulated preclinical diets to confirm correct levels of CA phytochemical markers. |
DOI | 10.1016/j.jcoa.2023.100091 |
Alternate Journal | J Chromatogr Open |
PubMed ID | 37789901 |
PubMed Central ID | PMC10544816 |
Grant List | R61 AT009628 / AT / NCCIH NIH HHS / United States S10 OD026922 / OD / NIH HHS / United States T32 AT002688 / AT / NCCIH NIH HHS / United States U19 AT010829 / AT / NCCIH NIH HHS / United States |