Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction.

Title	Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction.
Publication Type	Journal Article
Year of Publication	2010
Authors	Taylor AW, Traber MG
Journal	Anal Biochem
Volume	396
Issue	2
Pagination	319-21
Date Published	2010 Jan 15
ISSN	1096-0309
Keywords	Chromatography, High Pressure Liquid, Dinoprost, F2-Isoprostanes, Lipid Peroxidation, Solid Phase Extraction, Stereoisomerism, Tandem Mass Spectrometry
Abstract	F(2)-isoprostanes are stereo- and regioisomers of prostaglandin F(2alpha) (PGF(2alpha)) and are used as biomarkers for lipid peroxidation. We modified our liquid-liquid extraction (LLE) procedure for F(2)-isoprostane analysis (Anal. Biochem. 350 (2006) 41-51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC-tandem mass spectrometry (HPLC-MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF(2alpha).
DOI	10.1016/j.ab.2009.09.042
Alternate Journal	Anal. Biochem.
PubMed ID	19788885

Linus Pauling Institute