OREGON STATE UNIVERSITY
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| Title | Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction. |
| Publication Type | Journal Article |
| Year of Publication | 2010 |
| Authors | Taylor AW, Traber MG |
| Journal | Anal Biochem |
| Volume | 396 |
| Issue | 2 |
| Pagination | 319-21 |
| Date Published | 2010 Jan 15 |
| ISSN | 1096-0309 |
| Keywords | Chromatography, High Pressure Liquid, Dinoprost, F2-Isoprostanes, Lipid Peroxidation, Solid Phase Extraction, Stereoisomerism, Tandem Mass Spectrometry |
| Abstract | F(2)-isoprostanes are stereo- and regioisomers of prostaglandin F(2alpha) (PGF(2alpha)) and are used as biomarkers for lipid peroxidation. We modified our liquid-liquid extraction (LLE) procedure for F(2)-isoprostane analysis (Anal. Biochem. 350 (2006) 41-51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC-tandem mass spectrometry (HPLC-MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF(2alpha). |
| DOI | 10.1016/j.ab.2009.09.042 |
| Alternate Journal | Anal. Biochem. |
| PubMed ID | 19788885 |