|Title||Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Taylor AW, Traber MG|
|Date Published||2010 Jan 15|
|Keywords||Chromatography, High Pressure Liquid, Dinoprost, F2-Isoprostanes, Lipid Peroxidation, Solid Phase Extraction, Stereoisomerism, Tandem Mass Spectrometry|
F(2)-isoprostanes are stereo- and regioisomers of prostaglandin F(2alpha) (PGF(2alpha)) and are used as biomarkers for lipid peroxidation. We modified our liquid-liquid extraction (LLE) procedure for F(2)-isoprostane analysis (Anal. Biochem. 350 (2006) 41-51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC-tandem mass spectrometry (HPLC-MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF(2alpha).
|Alternate Journal||Anal. Biochem.|