Title | Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Taylor AW, Traber MG |
Journal | Anal Biochem |
Volume | 396 |
Issue | 2 |
Pagination | 319-21 |
Date Published | 2010 Jan 15 |
ISSN | 1096-0309 |
Keywords | Chromatography, High Pressure Liquid, Dinoprost, F2-Isoprostanes, Lipid Peroxidation, Solid Phase Extraction, Stereoisomerism, Tandem Mass Spectrometry |
Abstract | F(2)-isoprostanes are stereo- and regioisomers of prostaglandin F(2alpha) (PGF(2alpha)) and are used as biomarkers for lipid peroxidation. We modified our liquid-liquid extraction (LLE) procedure for F(2)-isoprostane analysis (Anal. Biochem. 350 (2006) 41-51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC-tandem mass spectrometry (HPLC-MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF(2alpha). |
DOI | 10.1016/j.ab.2009.09.042 |
Alternate Journal | Anal. Biochem. |
PubMed ID | 19788885 |