TitleThe secretory phenotype of senescent astrocytes isolated from Wistar newborn rats changes with anti-inflammatory drugs, but does not have a short-term effect on neuronal mitochondrial potential.
Publication TypeJournal Article
Year of Publication2018
AuthorsMaciel-Barón LÁngel, Morales-Rosales SLizbeth, Silva-Palacios A, Rodríguez-Barrera RHaydee, García-Álvarez JAntonio, Luna-López A, Pérez VIsabel, Torres C, Königsberg M
Date Published2018 10
KeywordsAnimals, Animals, Newborn, Anti-Inflammatory Agents, Astrocytes, Cellular Senescence, Central Nervous System, Dehydroepiandrosterone, Inflammation, Interleukin-1alpha, Isothiocyanates, Membrane Potential, Mitochondrial, Models, Animal, Neurons, Oxidative Stress, Rats, Rats, Wistar

In the central nervous system (CNS), senescent astrocytes have been associated with neurodegeneration. Senescent cells secrete a complex mixture of pro-inflammatory factors, which are collectively called Senescence Associated Secretory Phenotype (SASP). The SASP components can vary depending on the cell type, senescence inducer and time. The SASP has been mainly studied in fibroblasts and epithelial cells, but little is known in the context of the CNS. Here, the SASP profile in senescent astrocytes isolated from Wistar newborn rats induced to senescence by oxidative stress or by proteasome inhibition was analyzed. Senescent astrocytes secreted predominantly chemokines and IL-1α, but no IL-6. The effect of the anti-inflammatory drugs, sulforaphane (SFN) and dehydroepiandrosterone (DHEA), on the SASP profile was evaluated. Our results showed that SFN and DHEA decreased IL-1α secretion while increasing IL-10, thus modifying the SASP to a less anti-inflammatory profile. Primary neurons were subjected to the conditioned media obtained from drug-treated senescent astrocytes, and their mitochondrial membrane potential was evaluated.

Alternate JournalBiogerontology
PubMed ID30097900
Grant ListFON.INST/298/2016 / / CONACYT / International
Red Temática de Investigación en Salud y Desarrollo Social / / CONACYT / International