Title | Simultaneous, untargeted metabolic profiling of polar and nonpolar metabolites by LC-Q-TOF mass spectrometry. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Kirkwood JS, Maier C, Stevens JF |
Journal | Curr Protoc Toxicol |
Volume | Chapter 4 |
Pagination | Unit4.39 |
Date Published | 2013 May |
ISSN | 1934-9262 |
Keywords | Animals, Chromatography, High Pressure Liquid, Flavonoids, Metabolomics, Mice, Muscle, Skeletal, Propiophenones, Rats, Research Design, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Xenobiotics, Zebrafish |
Abstract | At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating this task. Performing multiple sample extractions, concentrating sample extracts, and using several separation and detection methods are common strategies to overcome these challenges but require a great amount of resources. This protocol describes the untargeted, metabolic profiling of polar and nonpolar metabolites with a single extraction and using a single analytical platform. |
DOI | 10.1002/0471140856.tx0439s56 |
Alternate Journal | Curr Protoc Toxicol |
PubMed ID | 23670865 |
PubMed Central ID | PMC3707319 |
Grant List | P30ES000210 / ES / NIEHS NIH HHS / United States S10RR027878 / RR / NCRR NIH HHS / United States R21 AT005294 / AT / NCCIH NIH HHS / United States S10 RR027878 / RR / NCRR NIH HHS / United States R21AT005294 / AT / NCCIH NIH HHS / United States P30 ES000210 / ES / NIEHS NIH HHS / United States |