TitleSimultaneous, untargeted metabolic profiling of polar and nonpolar metabolites by LC-Q-TOF mass spectrometry.
Publication TypeJournal Article
Year of Publication2013
AuthorsKirkwood JS, Maier C, Stevens JF
JournalCurr Protoc Toxicol
VolumeChapter 4
PaginationUnit4.39
Date Published2013 May
ISSN1934-9262
KeywordsAnimals, Chromatography, High Pressure Liquid, Flavonoids, Metabolomics, Mice, Muscle, Skeletal, Propiophenones, Rats, Research Design, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Xenobiotics, Zebrafish
Abstract

At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating this task. Performing multiple sample extractions, concentrating sample extracts, and using several separation and detection methods are common strategies to overcome these challenges but require a great amount of resources. This protocol describes the untargeted, metabolic profiling of polar and nonpolar metabolites with a single extraction and using a single analytical platform.

DOI10.1002/0471140856.tx0439s56
Alternate JournalCurr Protoc Toxicol
PubMed ID23670865
PubMed Central IDPMC3707319
Grant ListP30ES000210 / ES / NIEHS NIH HHS / United States
S10RR027878 / RR / NCRR NIH HHS / United States
R21 AT005294 / AT / NCCIH NIH HHS / United States
S10 RR027878 / RR / NCRR NIH HHS / United States
R21AT005294 / AT / NCCIH NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States