TitleSingle-Cell Migration as Studied by Scanning Electrochemical Microscopy.
Publication TypeJournal Article
Year of Publication2015
AuthorsJ Ummadi G, Joshi VS, Gupta PR, Indra AK, Koley D
JournalAnal Methods
Volume7
Issue20
Pagination8826-8831
Date Published2015 Oct 21
ISSN1759-9660
Abstract

Scanning electrochemical microscopy (SECM) was used to study the migration of single live head and neck cancer cells (SCC25). The newly developed graphite paste ultramicroelectrode (UME) showed significantly less fouling in comparison to a 10 μm Pt-UME and thus could be used to monitor and track the migration pattern of a single cell. We also used SECM probe scan curves to measure the morphology (height and diameter) of a single live cancer cell during cellular migration and determined these dimensions to be 11 ± 4 μm and 40 ± 10 μm, respectively. The migration study revealed that cells within the same cell line had a heterogeneous migration pattern (migration and stationary) with an estimated migration speed of 8 ± 3 μm/h. However, serum-starved synchronized cells of the same line were found to have a non-heterogeneous cellular migration pattern with a speed of 9 ± 3 μm/h. Thus, this non-invasive SECM-based technique could potentially be expanded to other cell lines to study cellular biomechanics for improved understanding of the structure-function relationship at the level of a single cell.

DOI10.1039/C5AY01944C
Alternate JournalAnal Methods
PubMed ID26528375
PubMed Central IDPMC4627705
Grant ListR01 AR056008 / AR / NIAMS NIH HHS / United States