TitleSynthesis of peroxynitrite from nitrite and hydrogen peroxide.
Publication TypeJournal Article
Year of Publication2005
AuthorsRobinson KM, Beckman JS
JournalMethods Enzymol
Date Published2005
KeywordsHydrogen Peroxide, Manganese Compounds, Mass Spectrometry, Nitrites, Oxides, Peroxynitrous Acid

We report a simple method for the synthesis of peroxynitrite from nitrite and hydrogen peroxide that can generate hundreds of milliliters of 180 mM peroxynitrite within 1 h from start to finish. It can be scaled down to make small quantities of isotope-labeled peroxynitrite. The method requires only a syringe pump and tubing connectors and is feasible for any biochemical laboratory. Unreacted hydrogen peroxide is eliminated with manganese dioxide, using an improved preparation compared to commercially available manganese dioxide. A number of contaminants were detected by mass spectrometry in peroxynitrite solutions cleaned with commercially purchased manganese dioxide. Nitrite contamination of the peroxynitrite solution is less than 2% as determined using the Griess method. The residual contaminants are principally 0.28 M sodium chloride and 0.1 M sodium hydroxide, which pose few problems when peroxynitrite is diluted for use in biological experiments.

Alternate JournalMeth. Enzymol.
PubMed ID16291234
Grant ListES00210 / ES / NIEHS NIH HHS / United States
P01 AT002034 / AT / NCCIH NIH HHS / United States
P01 ES00040 / ES / NIEHS NIH HHS / United States
R01 NS033291 / NS / NINDS NIH HHS / United States