TitleVitamin C supplementation lowers urinary levels of 4-hydroperoxy-2-nonenal metabolites in humans.
Publication TypeJournal Article
Year of Publication2011
AuthorsKuiper HC, Bruno RS, Traber MG, Stevens JF
JournalFree Radic Biol Med
Volume50
Issue7
Pagination848-53
Date Published2011 Apr 01
ISSN1873-4596
KeywordsAcetylcysteine, Administration, Oral, Adolescent, Adult, Aldehydes, Alkenes, Antioxidants, Ascorbic Acid, Biomarkers, Cross-Over Studies, Double-Blind Method, Female, Humans, Lipid Peroxidation, Male, Oxidation-Reduction, Oxidative Stress, Sex Factors, Smoking, Young Adult
Abstract

The lack of suitable biomarkers of oxidative stress is a common problem for antioxidant intervention studies in humans. We evaluated the efficacy of vitamin C supplementation in decreasing biomarkers of lipid peroxidation in nonsmokers and in cigarette smokers, a commonly studied, free-living human model of chronic oxidative stress. Participants received ascorbic acid (500mg twice per day) or placebo for 17 days in a double-blind, placebo-controlled, randomized crossover design study. The urinary biomarkers assessed and reported herein are derived from 4-hydroperoxy-2-nonenal (HPNE) and include the mercapturic acid (MA) conjugates of 4-hydroxy-2(E)-nonenal (HNE), 1,4-dihydroxy-2(E)-nonene (DHN), and 4-oxo-2(E)-nonenol(ONO). Vitamin C supplementation decreased the urinary concentrations of both ONO-MA (p=0.0013) and HNE-MA (p=0.0213) by ~30%; however, neither cigarette smoking nor sex affected these biomarkers. In contrast, vitamin C supplementation decreased urinary concentrations of DHN-MA (three-way interaction p=0.0304) in nonsmoking men compared with nonsmoking women (p<0.05), as well as in nonsmoking men compared with smoking men (p<0.05). Vitamin C supplementation also decreased (p=0.0092) urinary total of metabolites by ~20%. Thus, HPNE metabolites can be reduced favorably in response to improved plasma ascorbic acid concentrations, an effect due to ascorbic acid antioxidant function.

DOI10.1016/j.freeradbiomed.2011.01.004
Alternate JournalFree Radic. Biol. Med.
PubMed ID21236333
PubMed Central IDPMC3046321
Grant ListR01DK059576 / DK / NIDDK NIH HHS / United States
S10 RR022589 / RR / NCRR NIH HHS / United States
R01 DK067930 / DK / NIDDK NIH HHS / United States
R01DK067930 / DK / NIDDK NIH HHS / United States
R01 DK059576-05 / DK / NIDDK NIH HHS / United States
P30ES000210 / ES / NIEHS NIH HHS / United States
P30 ES000210-42 / ES / NIEHS NIH HHS / United States
S10RR022589 / RR / NCRR NIH HHS / United States
R01 HL081721 / HL / NHLBI NIH HHS / United States
R01 DK067930-04 / DK / NIDDK NIH HHS / United States
S10 RR022589-01 / RR / NCRR NIH HHS / United States
R01 DK081761 / DK / NIDDK NIH HHS / United States
R01 HL081721-05 / HL / NHLBI NIH HHS / United States
R01 DK059576 / DK / NIDDK NIH HHS / United States
R01HL081721 / HL / NHLBI NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States