TitleVitamin E deficiency decreases long-chain PUFA in zebrafish (Danio rerio).
Publication TypeJournal Article
Year of Publication2011
AuthorsLebold KM, Jump DB, Miller GW, Wright CL, Labut EM, Barton CL, Tanguay RL, Traber MG
JournalJ Nutr
Date Published2011 Dec
Keywordsalpha-Linolenic Acid, alpha-Tocopherol, Animals, Arachidonic Acid, Diet, Docosahexaenoic Acids, Fatty Acids, Unsaturated, Female, Gene Expression Regulation, Linoleic Acid, Lipid Peroxidation, Male, Real-Time Polymerase Chain Reaction, RNA, RNA, Messenger, Vitamin E Deficiency, Zebrafish

α-Tocopherol is a required, lipid-soluble antioxidant that protects PUFA. We hypothesized that α-tocopherol deficiency in zebrafish compromises PUFA status. Zebrafish were fed for 1 y either an α-tocopherol-sufficient (E+; 500 mg α-tocopherol/kg) or -deficient (E-; 1.1 mg α-tocopherol/kg) diet containing α-linolenic (ALA) and linoleic (LA) acids but without arachidonic acid (ARA), EPA, or DHA. Vitamin E deficiency in zebrafish decreased by ~20% (n-6) (P < 0.05) and (n-3) (P < 0.05) PUFA and increased the (n-6):(n-3) PUFA ratio (P < 0.05). In E- compared to E+ females, long chain-PUFA status was impaired, as assessed by a ~60% lower DHA:ALA ratio (P < 0.05) and a ~50% lower ARA:LA ratio (P < 0.05). fads2 (P < 0.05) and elovl2 (P < 0.05) mRNA expression was doubled in E- compared to E+ fish. Thus, inadequate vitamin E status led to a depletion of PUFA that may be a result of either or both increased lipid peroxidation and an impaired ability to synthesize sufficient PUFA, especially (n-3) PUFA.

Alternate JournalJ. Nutr.
PubMed ID22013196
PubMed Central IDPMC3223870
Grant ListHD062109 / HD / NICHD NIH HHS / United States
R01 DK043220 / DK / NIDDK NIH HHS / United States
R01 HD062109 / HD / NICHD NIH HHS / United States
DK43220 / DK / NIDDK NIH HHS / United States
RC4 ES019764 / ES / NIEHS NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States