Title | Vitamin E deficiency decreases long-chain PUFA in zebrafish (Danio rerio). |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Lebold KM, Jump DB, Miller GW, Wright CL, Labut EM, Barton CL, Tanguay RL, Traber MG |
Journal | J Nutr |
Volume | 141 |
Issue | 12 |
Pagination | 2113-8 |
Date Published | 2011 Dec |
ISSN | 1541-6100 |
Keywords | alpha-Linolenic Acid, alpha-Tocopherol, Animals, Arachidonic Acid, Diet, Docosahexaenoic Acids, Fatty Acids, Unsaturated, Female, Gene Expression Regulation, Linoleic Acid, Lipid Peroxidation, Male, Real-Time Polymerase Chain Reaction, RNA, RNA, Messenger, Vitamin E Deficiency, Zebrafish |
Abstract | α-Tocopherol is a required, lipid-soluble antioxidant that protects PUFA. We hypothesized that α-tocopherol deficiency in zebrafish compromises PUFA status. Zebrafish were fed for 1 y either an α-tocopherol-sufficient (E+; 500 mg α-tocopherol/kg) or -deficient (E-; 1.1 mg α-tocopherol/kg) diet containing α-linolenic (ALA) and linoleic (LA) acids but without arachidonic acid (ARA), EPA, or DHA. Vitamin E deficiency in zebrafish decreased by ~20% (n-6) (P < 0.05) and (n-3) (P < 0.05) PUFA and increased the (n-6):(n-3) PUFA ratio (P < 0.05). In E- compared to E+ females, long chain-PUFA status was impaired, as assessed by a ~60% lower DHA:ALA ratio (P < 0.05) and a ~50% lower ARA:LA ratio (P < 0.05). fads2 (P < 0.05) and elovl2 (P < 0.05) mRNA expression was doubled in E- compared to E+ fish. Thus, inadequate vitamin E status led to a depletion of PUFA that may be a result of either or both increased lipid peroxidation and an impaired ability to synthesize sufficient PUFA, especially (n-3) PUFA. |
DOI | 10.3945/jn.111.144279 |
Alternate Journal | J. Nutr. |
PubMed ID | 22013196 |
PubMed Central ID | PMC3223870 |
Grant List | HD062109 / HD / NICHD NIH HHS / United States R01 DK043220 / DK / NIDDK NIH HHS / United States R01 HD062109 / HD / NICHD NIH HHS / United States DK43220 / DK / NIDDK NIH HHS / United States RC4 ES019764 / ES / NIEHS NIH HHS / United States P30 ES000210 / ES / NIEHS NIH HHS / United States |