TitleXanthohumol improves dysfunctional glucose and lipid metabolism in diet-induced obese C57BL/6J mice.
Publication TypeJournal Article
Year of Publication2016
AuthorsMiranda CL, Elias VD, Hay JJ, Choi J, Reed RL, Stevens JF
JournalArch Biochem Biophys
Volume599
Pagination22-30
Date Published2016 06 01
ISSN1096-0384
KeywordsAnimals, Blood Glucose, Carbohydrate Metabolism, Cholesterol, LDL, Dietary Fats, Flavonoids, Humulus, Insulin, Interleukin-6, Leptin, Lipid Metabolism, Liver, Male, Mice, Obesity, Propiophenones, Proprotein Convertase 9
Abstract

Xanthohumol (XN) is a prenylated flavonoid found in hops (Humulus lupulus) and beer. The dose-dependent effects of XN on glucose and lipid metabolism in a preclinical model of metabolic syndrome were the focus of our study. Forty-eight male C57BL/6J mice, 9 weeks of age, were randomly divided into three XN dose groups of 16 animals. The mice were fed a high-fat diet (60% kcal as fat) supplemented with XN at dose levels of 0, 30, or 60 mg/kg body weight/day, for 12 weeks. Dietary XN caused a dose-dependent decrease in body weight gain. Plasma levels of glucose, total triglycerides, total cholesterol, and MCP-1 were significantly decreased in mice on the 60 mg/kg/day treatment regimen. Treatment with XN at 60 mg/kg/day resulted in reduced plasma LDL-cholesterol (LDL-C), IL-6, insulin and leptin levels by 80%, 78%, 42%, and 41%, respectively, compared to the vehicle control group. Proprotein Convertase Subtilisin Kexin 9 (PCSK-9) levels were 44% lower in the 60 mg/kg dose group compared to the vehicle control group (p ≤ 0.05) which may account for the LDL-C lowering activity of XN. Our results show that oral administration of XN improves markers of systemic inflammation and metabolic syndrome in diet-induced obese C57BL/6J mice.

DOI10.1016/j.abb.2016.03.008
Alternate JournalArch. Biochem. Biophys.
PubMed ID26976708
PubMed Central IDPMC4875845
Grant ListR01 AT009168 / AT / NCCIH NIH HHS / United States
S10 RR022589 / RR / NCRR NIH HHS / United States
S10 RR027878 / RR / NCRR NIH HHS / United States