TitleXanthohumol improves dysfunctional glucose and lipid metabolism in diet-induced obese C57BL/6J mice.
Publication TypeJournal Article
Year of Publication2016
AuthorsMiranda CL, Elias VD, Hay JJ, Choi J, Reed RL, Stevens JF
JournalArch Biochem Biophys
Date Published2016 06 01
KeywordsAnimals, Blood Glucose, Carbohydrate Metabolism, Cholesterol, LDL, Dietary Fats, Flavonoids, Humulus, Insulin, Interleukin-6, Leptin, Lipid Metabolism, Liver, Male, Mice, Obesity, Propiophenones, Proprotein Convertase 9

Xanthohumol (XN) is a prenylated flavonoid found in hops (Humulus lupulus) and beer. The dose-dependent effects of XN on glucose and lipid metabolism in a preclinical model of metabolic syndrome were the focus of our study. Forty-eight male C57BL/6J mice, 9 weeks of age, were randomly divided into three XN dose groups of 16 animals. The mice were fed a high-fat diet (60% kcal as fat) supplemented with XN at dose levels of 0, 30, or 60 mg/kg body weight/day, for 12 weeks. Dietary XN caused a dose-dependent decrease in body weight gain. Plasma levels of glucose, total triglycerides, total cholesterol, and MCP-1 were significantly decreased in mice on the 60 mg/kg/day treatment regimen. Treatment with XN at 60 mg/kg/day resulted in reduced plasma LDL-cholesterol (LDL-C), IL-6, insulin and leptin levels by 80%, 78%, 42%, and 41%, respectively, compared to the vehicle control group. Proprotein Convertase Subtilisin Kexin 9 (PCSK-9) levels were 44% lower in the 60 mg/kg dose group compared to the vehicle control group (p ≤ 0.05) which may account for the LDL-C lowering activity of XN. Our results show that oral administration of XN improves markers of systemic inflammation and metabolic syndrome in diet-induced obese C57BL/6J mice.

Alternate JournalArch. Biochem. Biophys.
PubMed ID26976708
PubMed Central IDPMC4875845
Grant ListR01 AT009168 / AT / NCCIH NIH HHS / United States
S10 RR022589 / RR / NCRR NIH HHS / United States
S10 RR027878 / RR / NCRR NIH HHS / United States