|Title||Zinc deficiency enhanced inflammatory response by increasing immune cell activation and inducing IL6 promoter demethylation.|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Wong CP, Rinaldi NA, Ho E|
|Journal||Mol Nutr Food Res|
|Date Published||2015 May|
|Keywords||Aging, Animals, Cells, Cultured, DNA Methylation, Female, Humans, Inflammation, Intercellular Adhesion Molecule-1, Interleukin-6, Macrophage Activation, Macrophages, Mice, Mice, Inbred C57BL, Promoter Regions, Genetic, Zinc|
SCOPE: Zinc deficiency results in immune dysfunction and promotes systemic inflammation. The objective of this study was to examine the effects of zinc deficiency on cellular immune activation and epigenetic mechanisms that promote inflammation. This work is potentially relevant to the aging population given that age-related immune defects, including chronic inflammation, coincide with declining zinc status.
METHODS AND RESULTS: An in vitro cell culture system and the aged mouse model were used to characterize immune activation and DNA methylation profiles that may contribute to the enhanced proinflammatory response mediated by zinc deficiency. Zinc deficiency upregulated cell activation markers ICAM1, MHC class II, and CD86 in THP1 cells, which coincided with increased IL1β and IL6 responses following LPS stimulation. A decreased zinc status in aged mice was similarly associated with increased ICAM1 and IL6 gene expression. Reduced IL6 promoter methylation was observed in zinc-deficient THP1 cells, as well as in aged mice and human lymphoblastoid cell lines derived from aged individuals.
CONCLUSION: Zinc deficiency induced inflammatory response in part by eliciting aberrant immune cell activation and altered promoter methylation. Our results suggested potential interactions between zinc status, epigenetics, and immune function, and how their dysregulation could contribute to chronic inflammation.
|Alternate Journal||Mol Nutr Food Res|
|PubMed Central ID||PMC4425307|
|Grant List||P30 ES000210 / ES / NIEHS NIH HHS / United States |
R21 ES023937 / ES / NIEHS NIH HHS / United States
P30 ES00210 / ES / NIEHS NIH HHS / United States